Peptide library

描述

Peptide Library Construction Service Features:

• Peptide library crude, we guarantee the purity of > 30%. Crude purity can also be as high as 75%, depending on the nature of the peptide sequence itself.
• Peptide library purity, according to customer needs, we can provide different levels of purity: > 70%, > 80%, even 98%.
• Each polypeptide is 5-20aa in length.
• Peptide modification includes fluorescent labeling, biotin labeling and D-amino acids.
• The minimum starting quantity of the peptide library is 48 peptides.
• General delivery time is 2-3 weeks. The peptide library was directly lyophilized in 96-well plates, which could be directly used in subsequent experiments.
• Strict quality control: Each peptide product provides MS and HPLC identification results.
• Synthesis quantity: The monthly flux of the biopeptide library can reach 8000-10000 pieces
• Technical support: Polypeptide technical support is permanent, and customers enjoy one-to-one service from experienced technical experts.

 

Peptide library type

Overlapping Peptide Library： It is mainly used for whole protein scanning. The polypeptide matrix is a common method for screening linear or continuous epitopes. The design of this peptide library is mainly determined by two key parameters: peptide length (peptide length) and step (offset number). Starting from the N-terminal and gradually intercepting the design to the C-terminal, it is likely that a solitary peptide (Orphan peptide) will be left at the end of the design. We suggest that the N-terminal transfer one or more amino acids to maintain the same length.

 

Truncation Peptide Library：Used to determine the minimum range of epitopes. The method of constructing truncated polypeptide matrix is to systematically remove amino acids on both sides of the original polypeptide sequence. The cephalopeptide matrices were used to identify the least amino acid sequences associated with activities.

 

Alanine Peptide Scanning Library：It is used to identify the effect of polypeptide on biological activity. Ala is systematically and sequentially substituted for each amino acid in the polypeptide sequence. The peptide library is screened by Ala to identify the effect of a particular amino acid on the structure, function and other biological activities of the whole protein.

 

Random Peptide Library：The selected amino acid residues were randomly and simultaneously replaced by 20 natural amino acids by shotgun approach. Zexi source random peptide library constructed as many mutations as possible in the selected amino acids. The alternative peptides in such libraries may enhance the activity of peptides.

 

Scrambled Peptide Library：The library is based on the internal substitution of the original polypeptide amino acid sequence. It is the most mutated peptide library, and scrambled peptides are often used as negative controls to confirm that a particular sequence is the key to protein function or activity. It is also a random screening tool to discover new targets.

 

Positional Scanning Peptide Library：For sequence modification to enhance peptide activity. Peptide libraries, where a selected region or site of a peptide is systematically replaced with other amino acids, help researchers discover specific regions that have a particular effect or activity at a particular location.