Resin is a derivative of styrene-divinylbenzene copolymer, is a kind of resin with acid-sensitive functional groups, which is widely used to prepare various amino compounds and their derivatives, and can also be used as solid phase support to synthesize polypeptides containing C-terminal amino groups.
This method has the advantages of mild reaction, less side effect and high yield. It can be used to remove peptides from the resin with trifluoro trifluoride / dichloromethane (DCM), avoiding the side effect of amino-acylation caused by the synthesis of strong acids (such as liquid hydrogen) with other carrier resins.
Since the resin itself has FMOC groups, the bonding method between one amino acid and the resin is the same as the bonding method of other subsequent amino acids (most solid phase synthesis carriers require a special process to link with one amino acid), which is very advantageous for automated production and therefore very popular.
The selection of solid phase carriers plays a very important role in solid phase peptide synthesis, It is not only related to the speed of peptide synthesis, but also has an important impact on the purity and yield of the target peptide, and has an important impact on the industrial production cost of the peptide. The correct choice of carrier is the key to the success of solid-phase synthesis.
The degree of substitution, degree of swelling and the length of peptide chain have effect on the yield of synthetic peptide. The diameter of resin, degree of cross-linking and degree of substitution have effect on the degree of swelling.
