Polypeptides are bioactive substances that involve a variety of cellular functions in organisms. It is a compound whose molecular structure lies between an amino acid and a protein. It is composed of a variety of amino acids in a certain order through peptide bonds. So far, more than 100 kinds of peptides have been found and isolated from human body, and the research and utilization of peptides has become a prosperous scene.
Peptide synthesis customization not only has very important theoretical significance, but also has important application value. Whole peptide synthesis can verify the structure of a new peptide, design a new peptide to study the relationship between structure and function, and provide important information for peptide biosynthesis mechanism, model enzyme and synthesis of new peptide drugs.
The technology of liquid and solid phase chemical synthesis of peptides has been mature. In recent decades, solid-phase peptide synthesis has become a conventional method for peptide synthesis because of its advantages of time-saving, labor-saving, material-saving, easy to be controlled by computer and easy to be popularized. It has expanded to other areas of organic matter, such as nucleotide synthesis. The basic principle and experimental process of solid phase synthesis are reviewed. The present situation of solid phase synthesis is analyzed. The development trend of solid phase synthesis is prospected.
Peptide synthesis is a process of repeated addition of amino acids, generally from the C-terminal (carboxyl end) to the N-terminal (amino end). In the past, peptide synthesis was carried out in solution. However, since Merrifield proposed solid-phase peptide synthesis in 1963, solid-phase synthesis has become a common technique for peptide and protein synthesis through continuous improvement and improvement, showing incomparable advantages over classical liquid-phase synthesis.
